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Quartet RNA reference materials were derived from immortalized EBV-infected B-LCLs from a quartet family, including monozygotic twin daughters (D5 and D6) and their father (F7) and mother (M8). Multi-batches of RNA-seq datasets were generated from independent laboratories using different library preparation protocols and sequencing platforms. Intra-batch proficiency and cross-batch reproducibility were then estimated. Based on multi-batches of RNA-seq data, we constructed ratio-based transcriptome-wide reference datasets and developed corresponding quality metrics.

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