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Schematic representation of the agoTRIBE method. The agoTRIBE approach fuses human Argonaute2 with the adenosine deaminase domain of human ADAR2, carrying a hyperactive mutation (E488Q) and depositing edits on the targeted transcripts. The edited nucleotides can be detected as A>G substitutions by either standard or single-cell RNA-seq. Top: schematic representation of the N-terminal tagging of human Argonaute2.

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