Heatmap from Scientific Research

ANI sensitivity to contamination and incompleteness. We took all pairs of MAGs with >99% ANI according to ANIm from species-level bins generated by Pasolli et al. 1 with >25 and <50 genomes, leading to a diverse set of 41,494 pairs of genomes. We re-evaluated the ANI of each method and performed ordinary least-squares regression with incompleteness and contamination (averaged between the pair and obtained by CheckM 22 ) as covariates. Estimated parameters and R 2 values are shown; only hexagons with >20 data points are visible (see Supplementary Fig. 4 for density information).

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