Workflow from Scientific Research

Schematic diagram of the initialization steps for the online calcium and voltage imaging algorithm. A set of initially acquired frames are used to estimate a reference template for motion correction; weighted masks representing spatial footprints of neurons and the neuropil (not shown); and parameters associated with deconvolution (calcium) and spike and subthreshold signal inference (voltage). Scale bars: a , 20 m (calcium imaging), 100 m (voltage imaging); c , 100 m.

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