Immunoblots of indicated proteins from lysates of HeLa cells treated with indicated concentrations of GSK’157 for 2.5 h. Active (A) and Inactive (I) GCN2 are indicated with arrows. Representative experiment from n = 4, biologically independent experiments. Ratio of P-eIF2α to eIF2α and ATF4 to Tubulin quantified from immunoblots and normalised to untreated cells. Data are shown as mean ± SEM (P-eIF2α n = 5 and ATF4 n = 4, except 0.02 and 0.04 μM compound treatment n = 4 and n = 3 respectively), biologically independent experiments. * p ≤ 0.0435, ** p ≤ 0.0038, *** p = 0.0002, as determined by one-way ANOVA with Dunnett’s multiple comparison test.
