Gel from Scientific Research

Caspase-1, GSDMD and IL-1 cleavage in infected macrophages and neutrophils from C57BL/6 and Nlrc4 -/- mice were examined by western blot. Macrophage westerns are combined whole cell lysate and TCA precipitated supernatants. Neutrophil whole cell lysates (WCL) and supernatants (SUP) were examined separately. Western blots are representative of 3 repeat experiments. Each data point in panels A-D represents one independent experiment (3-4 biological replicates). Statistical significance was assessed by 2-way ANOVA followed by Tukeys post-test. Error bars are mean +/- SD.

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