Workflow from Scientific Research

A P. aeruginosa T3SS is preassembled and spans the bacterial cell envelope (IM, inner membrane, OM, outer membrane and PG, peptidoglycan layer). Following cell contact, the pore-forming translocator proteins, PopB and PopD, assemble into a pore, which docks to the needle tip (PcrV, red). Effector secretion is triggered, resulting in export of effector proteins across the bacterial cell envelope and host cell plasma membrane (PM) into the cytosol of the targeted cell. P. aeruginosa strain PAO1 used in the current study expresses ExoS, ExoT, and ExoY, but not ExoU.

DOI