Schematic showing the interaction between 5HT2A and 5HT2B. In the violin plot in ( m – o ), the thick line in the middle is the median, the dotted lines are the interquartile range. In the box plots in ( b – d , g , h ) the line in the middle is plotted at the median. The box extends from the 25th to 75th percentiles. The whiskers indicate minimum and maximum values. Statistics: ns P > 0.05, * P ≤ 0.05, ** P ≤ 0.005, *** P ≤ 0.0005 from two-tailed Mann–Whitney test. P values in ( b ): 1 ( P = 0.006), 2 ( P = 0.006), 3 ( P = 3.23 × 10 −4 ), 4 ( P = 0.001), 5 ( P = 0.009), 6 ( P = 0.002). P values in ( c ): 1 ( P = 4.31 × 10 −4 ), 2 ( P = 0.024), 3 ( P = 7.5 × 10 −4 ). P values in ( d ): 1 ( P = 0.384), 2 ( P = 0.892), 3 ( P = 0.291). P values in ( g ): 1 ( P = 0.001), 2 ( P = 0.019), 3 ( P = 0.279), 4 ( P = 0.037), 5 ( P = 0.019), 6 ( P = 0.019), 7 ( P = 0.008), 8 ( P = 0.008), 9 ( P = 0.008), 10 ( P = 0.008), 11 ( P = 0.056), 12 ( P = 0.016), 13 ( P = 0.016), 14 ( P = 0.016), 15 ( P = 0.016), 16 ( P = 0.111), 17 ( P = 0.286), 18 ( P = 0.111), 19 ( P = 0.001), 20 ( P = 0.001), 21 ( P = 0.001), 22 ( P = 0.004), 23 ( P = 0.008), 24 ( P = 0.001), 25 ( P = 0.905), 26 ( P > 0.999), 27 ( P = 0.905), 28 ( P = 0.905), 29 ( P = 0.905), 30 ( P > 0.999). P values in ( h ): 1 ( P = 0.001), 2 ( P = 0.008), 3 ( P = 0.016), 4 ( P = 0.001), 5 ( P = 0.73). n = number of embryos. For sFCCS, n = number of independent measurements. Scale bars 5 µm. Source data are provided as a Source Data file.
