Medial MyoII intensities in different genotypes. In the box plots in ( c , d , f , i , j , k ), the line in the middle is plotted at the median. The box extends from the 25th to 75th percentiles. The whiskers indicate minimum and maximum values. Statistics: ns P > 0.05, * P ≤ 0.05, ** P ≤ 0.005, *** P ≤ 0.0005, **** P ≤ 0.00005 from two-tailed Mann–Whitney test. P values in ( c ): 1 ( P = 0.635), 2 ( P = 0.368), 3 ( P = 0.031), 4 ( P = 0.022), 5 ( P = 0.011), 6 ( P = 0.005), 7 ( P = 0.0007), 8 ( P = 0.0004), 9 ( P = 0.005), 10 ( P = 0.002), 11 ( P = 0.93), 12 ( P = 0.479), 13 ( P = 0.669), 14 ( P = 0.536), 15 ( P = 0.475), 16 ( P = 0.256), 17 ( P = 0.126), 18 ( P = 0.364), 19 ( P = 0.417), 20 ( P = 0.536). P values in ( d ): 1 ( P = 0.098), 2 ( P = 0.015), 3 ( P = 0.007), 4 ( P = 0.073), 5 ( P = 0.031), 6 ( P = 0.009), 7 ( P = 0.004), 8 ( P = 0.026), 9 ( P = 0.026), 10 ( P = 0.002), 11 ( P = 0.015), 12 ( P = 0.051), 13 ( P = 0.035), 14 ( P = 0.101), 15 ( P = 0.073), 16 ( P = 0.001), 17 ( P = 0.004), 18 ( P = 0.002), 19 ( P = 0.002), 20 ( P = 0.0002), 21 ( P = 0.818), 22 ( P = 0.628), 23 ( P = 0.534), 24 ( P = 0.445), 25 ( P = 0.181). P values in ( f ): 1 ( P = 0.012), 2 ( P = 0.0004), 3 ( P = 0.008), 4 ( P = 0.088), 5 ( P = 0.036), 6 ( P = 0.088), 7 ( P = 4.1 × 10 −5 ), 8 ( P = 1.65 × 10 −4 ), 9 ( P = 8.2 × 10 −5 ), 10 ( P = 2.88 × 10 −4 ), 11 ( P = 4.94 × 10 −4 ), 12 ( P = 4.94 × 10 −4 ), 13 ( P = 8.2 × 10 −5 ), 14 ( P = 8.2 × 10 −5 ), 15 ( P = 8.2 × 10 −5 ), 16 ( P = 1.65 × 10 −4 ), 17 ( P = 3.29 × 10 −4 ), 18 ( P = 8.2 × 10 −5 ). P values in ( i ): 1 ( P = 0.002), 2 ( P = 0.01), 3 ( P = 6.22 × 10 −4 ), 4 ( P = 0.005), 5 ( P = 0.002), 6 ( P = 0.003), 7 ( P = 0.001), 8 ( P = 0.005), 9 ( P = 0.442), 10 ( P = 0.505), 11 ( P = 0.235), 12 ( P = 0.2), 13 ( P = 0.021), 14 ( P = 0.021), 15 ( P = 0.003), 16 ( P = 0.05), 17 ( P = 0.005), 18 ( P = 0.002), 19 ( P = 0.005), 20 ( P = 0.002), 21 ( P = 0.574), 22 ( P = 0.442), 23 ( P = 0.234), 24 ( P = 0.195). P values in ( j ): 1 ( P = 0.021), 2 ( P = 0.021), 3 ( P = 0.328), 4 ( P = 0.13), 5 ( P = 0.328), 6 ( P = >0.999), 7 ( P = 0.798), 8 ( P = 0.878), 9 ( P = 0.959), 10 ( P = 0.005), 11 ( P = 0.234), 12 ( P = 0.105). P values in ( k ): 1 ( P = 3.11 × 10 −4 ), 2 ( P = 1.55 × 10 −4 ), 3 ( P = 1.55 × 10 −4 ), 4 ( P = 1.55 × 10 −4 ), 5 ( P = 0.002), 6 ( P = 0.001), 7 ( P = 0.002), 8 ( P = 0.001), 9 ( P = 0.083), 10 ( P = 0.028), 11 ( P = 0.105), 12 ( P = 0.234). n = number of embryos. Scale bars 5 µm. Source data are provided as a Source Data file.
