Quantification of cumulative T1 events in the respective conditions. The center dashed line is the mean and the error bands are the standard deviation. Statistical significance was calculated by two-tailed Mann–Whitney U test at 30 min (* P ≤ 0.05). In the box plots in ( e , f , g , i , j , k ), the line in the middle is plotted at the median. The box extends from the 25th to 75th percentiles. The whiskers indicate minimum and maximum values. Statistics: ns P > 0.05, * P ≤ 0.05, ** P ≤ 0.005, *** P ≤ 0.0005, **** P ≤ 0.00005 from two-tailed Mann–Whitney test. n = number of embryos. Scale bars in ( b , l ) 10 µm, in ( d , h ) 5 µm. Source data are provided as a Source Data file.
