A549 cells were pre-treated with compound at the indicated doses (2 hr) and infected with pH1N1 (MOI 0.5), H3N2 (MOI 0.5) or H5N1 (MOI 0.05) IAV for 24 hr. Percent IAV-infected cells were quantified by immunostaining for IAV NP followed by high throughput imaging (blue line=pH1N1; green line=H3N2; purple line=H5N1). Percent alive cells were quantified by MTT assay in uninfected A549 cells (black line). Data points represent the mean across n = 3 biologically independent samples. Schematics mark the target with corresponding PPI or PH dataset and IAV strain, and the corresponding compound (at left). Compounds are annotated with IC50 values for IAV strains in which SI > 2. Error bars represent standard error of mean (SEM). H Dose-response curves for members of the MAPK pathway (pathway schematic at left), including MAP2K3, MAP2K6, MAPK13 and MAPKAPK2, each annotated with corresponding compounds. PF-3644022 was not tested against pH1N1.
