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Recovery of luciferase denatured (42 °C, 40 min) in the presence of 1 µM DNAJA2, 2 µM Hsc70 or 0.4 µM Apg2. After denaturation, 2 mM ATP and the missing chaperone components were added to end in all cases with 1 µM DNAJB1 (grey), DNAJA2ΔCD (red) or DNAJA2wt (blue), 0.4 µM Apg2 and 2 µM Hsc70, and recovery was measured after 120 min at 30 °C. Data are mean ± SD of three independent experiments. Significance was evaluated using a two-tailed, one-way analysis of variance (ANOVA) and Tukey’s multiple comparison, and the p values are shown in ( a ), ( c – f ).
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