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Brains of surviving mice were inoculated orally into 2 recipient mice and infectivity was tested by in vitro cultivation ( e ), serum levels of IFNγ ( f ; D6 PI; N = 6 mice per group except negative controls which had N = 3) and serum anti- Toxoplasma antibodies ( g ; D49 PI; N = 2 for WT, 12 for ΔROCY1, 16 for ΔBFD1, 5 for + control and 6 for – control serum).

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