Line Plot from Scientific Research

Pulse carried out at 60 C using 100 mM Fmoc-piperidine (2), 5 mM p-nitrophenyl acetate (3), 1 M phenyl acetate (4) and 5 mM N-methylpiperidine (5) in DMSO. The reaction was followed by in situ infrared spectroscopy, with concentrations estimated using 1 H-NMR spectroscopy (details in Supplementary Information ): piperidine (1, green), Fmoc-piperidine (2, blue), DBF (6, red), PipAc (7, purple). The concentration of piperidine (1) was determined by taking the difference between the concentrations of DBF (6) and PipAc (7). Curves are an average of two experiments; for visual clarity, error bars (s.d.) are shown only for every 1 min. The pulse is divided into three distinct phases: a lag phase (I), exponential growth (II, starts at arrow A) and decay (III, starts at arrow B).

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