Heatmap showing the differential expression of transcription factors in 35–40 days Cas9-CTL and KO hSO examined by RT–qPCR ( n = 12–13, from seven differentiations and three hiPS cell lines); two-tailed Mann–Whitney test with Benjamini–Hochberg adjusted P value. Stars represent comparisons between Cas9-CTL and CSDE1 (left) or SMAD4 (right) KO. Data are presented as mean ± s.e.m. (standard error of mean, c , d , f , g ); one-way ANOVA ( c , F 3,57 = 78.64; d , F 2,83 = 20.70; g , F 2,111 = 38.74) or mixed model two-way ANOVA ( f , genotype as factor, F 2,81 = 45.29) using Dunnett’s multiple comparison test. **** P < 0.0001. Supplementary Table 5 shows sample size and P values for f and h .
