Bar Plot from Scientific Research

RT–qPCR quantification of polyadenylated (polyA + ) SOA mRNA isoform levels in females and males at larval (L1–L4), pupal and adult stages. The scheme (top) shows the primer strategy. Left, percentage unspliced relative to total (spliced and unspliced) mRNA levels. Right, percentage spliced mRNA relative to the average male spliced mRNA level at each stage. The bars represent the mean of n = 2 or n = 3 independent biological replicates indicated by overlaid data points. Rp49 was used for normalization (Extended Data Fig. 4c and raw data in Supplementary Table 1).

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