Oncolytic RSV-A2 Cytotoxicity on TC-1 cell lines assessed via LDH test. In 96-well plates, TC-1 tumor cells were seeded and treated with RSV-A2 at different MOIs for 72 h. The LDH data demonstrated that infections at MOIs of 5, 10, and 15 could significantly release LDH versus the control groups. However, TC-1 cancer cell lines treated with RSV-A2 at 15 MOI did not demonstrate statistically significant survivability than cultures inoculated at 10 MOI (P > 0.05). **** (P < 0.0001) represents a statistically significant difference between MOI 10 and MOI 5, as well as between the 5 and 1 groups by ANOVA(one-way).
