Mutations made to generate engineered variants depicted in the Fv region of the Fab of AB-000224 in complex with NPNA4 (gold ribbon, PDB ID 6WFY ; Methods ). The light-chain framework is shown in silver and the heavy-chain in pale gray. The CDRs for light and heavy chains are indicated, respectively, as CDR1 (light and dark blue), CDR2 (light and dark purple) and CDR3 (light and dark cyan). Mutation sites of engineered variants are labeled as stability violations (red), deamidation sites (green) and paired sibling sites (gray; Methods ).
