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Experimental scheme and ratios of CldU:IdU tract lengths in MEFs under unperturbed conditions or after exposure to 2 M APH or 0.5 mM HU for 4 h. At least 200 DNA fibers were scored per group. Three independent experiments were conducted. Data are shown as the mean SEM. Unpaired two-tailed Student’s t -test, ns, not significant, * P < 0.05 and *** P < 0.001

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