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Schematic diagram of reporter and effector in Arabidopsis protoplast transactivation assays. Reporter is the fusion of the CYP79D3, CYP736A2, and UGT85K3 promoter with firefly luciferase gene (LUC). The LjMTR, site mutated LjMTR-D230 and LjZCD fused with the CaMV 35S promoter respectively to from effectors. NOS, the transcriptional terminator of the nopaline synthase gene from Agrobacterium tumefaciens.
#Workflow#Illustration#Schematic Diagram#Reporter#Effector#Arabidopsis Protoplast#Transactivation Assays#CYP79D3#CYP736A2#UGT85K3#Luciferase#LjMTR#LjMTR-D230#LjZCD#CaMV 35S promoter#NOS
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