Mean ± SEM normalised MUA response to light steps targeting S- or L-cone opsins (left) or for both cone opsins with or without changes in brightness for melanopsin and rods (right) as a function of recording site depth within the SPZ ( n = 8–26 sites/bin). Data in A - D analysed by one-way RM ANOVA with Tukey’s post tests ( A : F 3, 12 = 12.4, P = 0.0006; B : F 3, 60 = 525.7, P < 0.0001; C : F 3, 57 = 15.4, P < 0.0001; D : F 3, 381 = 29.4, P < 0.0001). Data in E analysed by 2-way mixed effects ANOVA (left- Stim.: F 1,240 = 216.3, P < 0.0001; position— F 11,240 = 0.84, P = 0.596; Stim. X position— F 11,240 = 0.99, P = 0.459; right—Stim.: F 1,240 = 118, P < 0.0001; position- F 11, 240 = 0.803, P = 0.637; Stim. X position- F 11,240 = 1.05, P = 0.403). *,** and *** indicate P < 0.05, P < 0.01 and P < 0001, respectively
