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Phylogenetic trees of PCDA2 were constructed by 100 bootstraps and rooting at the midpoint, the leaf nodes with back, grey and light grey square mean obligate cleaner, facultative cleaner and non-cleaner, and the number of each gene showed according to the order as dedicated (L. dimidiatus), facultative (T. bifasciatum, S. melops, T. adspersus, S. pulcher, L. bergylta) and non-cleaners (C. undulatus, N. celidotus). The bold branches indicate the internal nodes with bootstraps 80

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