ELISA reactivity, SHM levels and vaccinee protection status of mAbs from expanded lineages (n = 349). Number of nucleotide mutations from germline (SHM) for mAbs that are not reactive (dark blue, n = 185), show an indeterminant, weak signal (orange, n = 29) or are reactive (light green, n = 135) in a CSP ELISA. Domain specificity for CSP-reactive mAbs is shown in the box with a dashed green outline. Monoclonal antibodies reactive by ELISA to the NANP6 repeat-region peptide (dark green, n = 98), to the C-terminal region peptide (Pfs16, royal blue, n = 20) or not reactive in either peptide ELISA (gray, n = 9). Lines represent median values, *** P < 0.0001, ** P < 0.001, unpaired two-tailed Mann–Whitney test. CSP-reactive mAbs that were not tested in peptide ELISAs (n = 8) are not shown.
