Microscopy from Scientific Research

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Timecourse of IAA or EtOH treatment for 12, 24, or 48 h to deplete SPT16, followed by immunocytochemistry showing a progressive reduction in the expression of OCT4. Images are representative of plate-wide immunofluorescence changes. Left panels are DAPI-stained (blue), while right panels show OCT4 immunofluorescence (green). OCT4 immunofluorescence is quantified in Additional File 1 : Fig. S1F
#Microscopy#Timecourse#IAA Treatment#SPT16 Depletion#Immunocytochemistry#OCT4 Expression#DAPI Staining#Immunofluorescence
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