Characteristics of predicted secreted proteins and their corresponding gene distribution on the 26 chromosomes in the cotton genome. The density data were calculated by the number of encoded genes using step windows (window = 500 kb, walking step = 100 kb). Secreted proteins were predicted as those with signal peptide (SP), lack of the transmembrane (TM) domain, and the extracellular location. The transmembrane domain was predicted by two tools, TMHMM2.0 [ 55 ] and Phobius [ 56 ]. The subcellular location of the prediction of secreted proteins was carried out using the plant model of the WolfPsort procedure by (circle e) [ 54 ], and the predicted localization during the hostpathogen interaction was predicted using the fungi model (circle gi). A01A13 and D01D13 represent the 13 chromosomes of the A subgenome and the D subgenome of the ZMM2 genome, respectively.