Validation experiment. A Validation experiment’s schematic figure. Step A―Predicting the recipient’s property post-FMT. Inserting all the MIPMLP preprocessed donors from all the cohorts (including donors that had not been actually transplanted) into the pre-trained iMic model, using existing datasets. iMic returns the predicted recipients’ properties post-FMT. Step B―Grouping samples for FMT validation experiments. Two groups were defined from the predicted properties. A group of predicted to be high (with predicted high values of the property) and predicted to be low (with predicted low values of the property). Step C―FMT experiment timeline. Two groups of mice were raised till the age of 6 weeks. They got antibiotic treatment (weeks 6–8). At the age of 8 weeks, stools were collected and sequenced. At the age of 8 weeks + 1 day, they received the first FMT. One group of mice got the FMT from the predicted to be high donors group, and the second group of mice got the FMT from the predicted to be low group. They got the second FMT from the same donors at the age of 9 weeks. Stool samples were collected a week after the second transplant at the age of 10 weeks and 6 weeks after the second FMT treatment at the age of 15 weeks. Step D―Recipient samples analysis. The real recipients’ properties were calculated from the mice’s stool samples at the age of 10 weeks. A comparison between the 2 groups’ properties showed significant differences in the targeted properties.
