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Schematic of experimental setup with 8 to 12-week-old male and female C57BL/6 J and Rag2 del mice. The sham group underwent two thoracotomies and an intramyocardial injection of Matrigel. The MI group underwent two thoracotomies, permanent LAD ligation, and intramyocardial injection of Matrigel. The MI-CM group underwent two thoracotomies, permanent LAD ligation, and intramyocardial injection of 1 million neonatal GFP cardiomyocytes suspended in Matrigel. Flow cytometric analysis of the cardiac immune response at D4 and D7 after the initial thoracotomy (post OP) or LAD ligation (post MI) was carried out.

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