Thousand grain weight (TGW) in WT and KO lines in the field. Data are shown as means SD ( n = 20). Triple KO line, KO-ABD; double KO line ( TraesCS7A02G246500 and TraesCS7D02G245200 KO line), KO-AD; double KO line ( TraesCS7A02G246500 and TraesCS7B02G144900 KO line), KO-AB; double KO line ( TraesCS7B02G144900 and TraesCS7D02G245200 KO line), KO-BD; single KO line ( TraesCS7A02G246500 KO line), KO-A; single KO line ( TraesCS7B02G144900 KO line), KO-B; single KO line ( TraesCS7D02G245200 KO line), KO-D. Different lowercase letters indicate significant differences ( P < 0.05), as determined by ANOVA
