Log 2 fold-change (LFC) values for reagents targeting the same gene are collapsed to gene means per cell line. Boxes represent the distribution of gene mean LFC across all cell lines for genes included in the nonessential control set [ 24 ] (738 genes), non-expressed set (10 randomly sampled genes from each cell line with RNAseq log 2 (TPM + 1) < .2), unbiased essential gene set described in the Methods (744 genes), or the core essential control set [ 24 ] (208 genes). CRISPR reagents are from the Avana library and RNAi reagents are those used in the DEMETER2-combined [ 16 ] RNAi dataset (union of Achilles and DRIVE libraries). Whiskers are extended to 4 times the interquartile range to limit overplotting outliers since over 1.2 million data points are represented.
