smFISH staining of indicated marker genes ( PAPPA , FLT1 and hCG) characterizes lSTB mature 1 and lSTB mature 2 in late pregnancy (left). Statistical analysis of the proportion of lSTB mature 1 and lSTB mature 2 (middle). Data are shown as mean ± s.d. P values by unpaired two-tailed t test. n = 6 donors. The schematic represents the distribution of lSTB mature 1-a, lSTB mature 1-b and lSTB mature 2 in the late placental STB (right).