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ELISA analysis of CSH2 expression in trophoblast organoids derived from hTSCs-BL with DOX-inducible overexpression of MITF. Data are shown as mean s.d. P values by unpaired two-tailed t test. n = 3 independent experiments. CDS, coding sequence; hPGK, human phosphoglycerate kinase 1 promoter; P2A: porcine teschovirus-1 2A peptide; Puro, puromycin; TRE3GS, TRE3GS inducible promoter; Tet-on 3G, Tet-on 3G transactivator protein; WPRE, Woodchuck hepatitis virus’s post-transcriptional regulatory element; OE, overexpression.
#Bar Plot#Error Bars#ELISA Analysis#CSH2 Expression#Trophoblast Organoids#hTSCs-BL#DOX-Inducible Overexpression#MITF#Independent Experiments#Coding Sequence#Human Phosphoglycerate Kinase 1 Promoter#Porcine Teschovirus-1 2A Peptide#Puromycin#TRE3GS Inducible Promoter#Tet-on 3G Transactivator Protein#Woodchuck Hepatitis Virus’s Post-Transcriptional Regulatory Element#Overexpression
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