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Schema of bioinformatic strategy to study the effect of BF10 within tumor microenvironment. Tumors derived from two syngeneic tumor models (HNSC/Q1-2 and BRCA/4T1) treated with BF10, IL-10-Fc, CSF-1R, or control were collected for bulk RNA-seq to investigate the differentially expressed genes (DEGs; >1.5-fold and p < 0.05 or <0.5-fold and p < 0.05). DEGs were analyzed using 2 modules: (1) Bioinformatics Database for Annotation, Visualization, and Integrated Discovery (DAVID;https://david.ncifcrf.gov/) and (2) Ingenuity Pathway Analysis (IPA).

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