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A schematic representation of the experimental outline for Figures 1, 2, and S4. CFSE-labeled OT-I CD45.1 T cells were adoptively transferred into control or DCICAM-1 mice; 18 h later mice were immunized with intrafootpad injection of DEC-205:OVA plus CD40, and OT-I activation (CD69 and CD25), DC-OT-I cell conjugate formation, and OT-I cell proliferation (CFSE) were assessed at the indicated time points.

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