Microscopy from Scientific Research

Infected THP-1 macrophages 3 h p.i. were imaged by immunofluorescence using an anti-LC3B polyclonal antibody. (Top) The QV306 AcVs do not co-localize with the autophagosome marker LC3B. (Bottom) (control) Vacuoles containing Burkholderia cenocepacia isolate DFA35 co-localize with LC3B. Images taken with 63 magnification on Leica SP8 confocal microscope. Multiplicity of infection, 80. Scale bar, 10 m.

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