IncuCyte proliferation analysis. HeLa cells maintained in a Dox-free culture medium were split into two groups (Dox+/-) for lentivirus infection, followed by transient transfection of rtTA-expression or control pcDNA3.1 plasmids 24 h after infection. GFP-positive cells were sorted by FACS for IncuCyte proliferation analysis. Error bars, means +/- s.d., n = 3 biologically independent experiments.
