Quantification of CXCR4lopre-cDC numbers in femur BM of naive or IAV-infected WT andClec9aCreCcr2fl-eGFPmice (n = 12–21 mice). cDC lineage cells inClec9aCreCcr2fl-eGFPmice express one WT and one targetedCcr2allele (C9aWTCCR2) or two targeted alleles (conditional knockouts for CCR2; C9aΔCCR2). Data in (C) and (E) are depicted as frequencies in Figures S4C and S4D. All IAV-infected mice were harvested at day 2 postinfection. Data in (A) and (D) are representative of at least two experiments and (B), (C), and (E) are pooled data from two (B and C) or six (E) independent experiments. Each dot in represents one mouse, bars represent averages, and error bars represent SD. In (D), each line represents one mouse. All statistics were calculated using two-way ANOVA. See alsoFigure S4.*p < 0.05,**p < 0.01, and***p < 0.001; ns, p > 0.05.
