Bar Plot from Scientific Research

Open access visualization of Bar Plot, Microscopy, Retinoid Distribution, RPE, Fluorescence Intensity Images
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Distribution of retinoids in the RPE of 2-month-old Rgr+/+ and RgrS/S mice. Two-photon-excited (740-nm) fluorescence intensity images and respective FLIM phasor plots (underneath) obtained for dark- and daylight-adapted eyes. Red circles in each phasor plot indicate location of the retinosome phasor points, and corresponding image pixels were pseudo-colored (red) in the intensity images. Scale bars: 25 m. The bar graph shows quantification of the areas occupied by retinosomes in Rgr+/+ and RgrS/S RPEs. Values are plotted as mean SEM;**p < 0.01,*p < 0.05 by Student’s t tests with Holm-Šídák correction; n = 4 (light), n = 3 (dark) independent experiments.

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