Workflow from Scientific Research

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MPRA hits were analyzed to determine whether they could be CRISPR base edited, then cells were transfected with corresponding base editors and sgRNAs to grow out single-cell clonal wild-type and 3' mutant cell lines confirmed by Sanger sequencing.
#Workflow#Bar Plot#Line Plot#Illustration#MPRA hits#CRISPR base edited#Cells#Base editors#sgRNAs#Single-cell clonal wild-type#3' mutant cell lines#Sanger sequencing
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