Line Plot from Scientific Research

Mutations in the double mutant. Top, schematic representations of theFLYC1(seeFigure 1B) andFLYC2genes (black boxes, exons; yellow, transmembrane regions). Purple asterisks mark the locations of the gRNA-targeted sites, g1 through g4. Bottom, Sanger sequencing of PCR products from genomic DNA template of wild-type and double-mutant plants, showing mutations on the two homologous chromosomes in the diploid genome. Residue positions are in the coding sequences relative to the +1 ATG sites. del, deletion; ins, insertion.

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