Line Plot from Scientific Research

Examples of mutations detected in theFLYC1gene at the g1 site. Top, schematic of theFLYC1gene, showing exons (black boxes), transmembrane-coding regions (yellow boxes), and region overlapping the g1 gRNA-targeted site (purple lines). Below, Sanger sequencing results of PCR products from genomic DNA template showing deleterious mutations at the g1 site in theFLYC1gene of the diploid Venus flytrap genome for different mutant lines. The region covering +1,054 to +1,076 of the gene is shown (relative to the +1 ATG start site). del, deletion; delins, deletion-insertion. All alleles result in frameshifts coding for predicted truncated forms of the FLYC1 protein lacking the pore domain (transmembrane region 6). For example, a deletion of a single G residue at position +1,069 (purple circle in wild-type allele) causes a frameshift mutation and premature stop codon (e.g., purple box in mutant line 1069del/1068_1069del). See alsoFigure S1.

DOI