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Schematic overview of the experimental design. Briefly, 11 gliomas induced by pCAG:PDGFB GFP-Barcode transduction were analyzed by RNA sequencing. RNA-seq data were used to retrieve both gene expression and barcode counts. Barcode counts were used to calculate Shannon entropy of each tumor and perform trait-associated gene expression analysis of gliomas. Genes were ranked by their correlation with Shannon entropy to perform GSEA analysis.

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