Left: snapshot of Ribo-seq (black) and Disome-seq (gray) reads mapped to the NET1 transcript. For Ribo-seq, reads are plotted to correspond to the approximate P-site of the ribosome. For Disome-seq, reads are plotted to correspond to the approximate P-site of the trailing ribosome in the disome complex. Stalling peaks are indicated with arrows. The amino acid and nucleotide sequence of the stalling motifs corresponding to E, P, A sites of the ribosome are shown below the snapshot, with the P-site amino acids underlined. Right: magnified snapshot of the Ribo-seq (black) and Disome-seq (gray) peaks within the region indicated by a red bracket in the left. Disome peaks ~28 nt (a typical ribosome footprint length) apart from each other and ~28 nt upstream of the first stalling site indicate the possibility of queued ribosomes in this region.
