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BRCA2-RAD51 complex is recruited to the proximity of DSBs. BRCA2 then either nucleates RAD51 directly on RPA-coated ssDNA or slides on the dsDNA arm. During sliding, proximal nucleosomes serve as a diffusion barrier, restricting the sliding of BRCA2 toward resected RPA-coated ssDNA. RAD51 is nucleated and stabilized on ssDNA by BRCA2 and grows into a filament.

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