Microscopy from Scientific Research

A representative kymograph showing diffusion-driven delivery of BRCA2-RAD51 complexes to ssDNA in the vicinity of the ds-ssDNA junction. Static BRCA2-eGFP molecules bound directly to the ssDNA gap or mobile BRCA2-eGFP molecules diffusing along dsDNA are indicated by arrows. 25 nM RAD51(A466) (red) was incubated with gDNA in the presence of 5 nM BRCA2-eGFP (blue) and 1.25 nM RPA in the presence of 5 nM SYTOX Orange. Position of the ssDNA gap is indicated by dashed lines.

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