Protein Structure from Scientific Research

RFU of PI(4,5)P2: S1P: SPNS2 compared withapoSPNS2 at 60 min labeling. The helices are colored according to the corresponding HDX results above. N and C domains are shown in green and yellow, respectively, with S1P and PI(4,5)P2, green and orange spheres. A greater deuteration of the helices on the extracellular side indicates PI(4,5)P2synergistically enhances S1P-induced opening of the extracellular gate while stabilizing the intracellular gate.

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