Scatter Plot from Scientific Research

Relationship between pegRNA RTT/PBS secondary structure and PE6d improvements. The y axis reflects the fold-improvement of PE6d over PEmaxΔRNaseH. The x axis is the absolute value of the free energy of pegRNA folding as measured by NUPACK. Each dot represents one edit in HEK293T cells that was calculated from the mean values from n = 3 biological replicates. SeeFigure S4D for individual editing values and edit identities.

DOI