Flowchart from Scientific Research

Summary of evolution campaigns for NpuC-Gs RT, NpuC-Ec48 RT, or NpuC-Tf1 RT phage in the v1 (yellow), v2 (blue), and v3 (purple) PE-PACE circuits. Whether an evolution was PANCE or PACE is specified. PANCE passages (p) or hours of PACE (h) are specified in parentheses. Arrowheads indicate increases in selection stringency. Mutants characterized in mammalian cells are denoted with a dot and labeled. Additional increases in stringency are in pink.

DOI