Illustration from Scientific Research

Schematic of PE-PACE selection circuit. Upon infection ofE. coliby selection phage (blue), the NpuN intein and NpuC intein (pink) mediate reconstitution of the PE2 prime editor (purple and pink), which engages a pegRNA (dark green) and corrects a frameshift in T7 RNAP (orange) via PE. Functional T7 RNAP then transcribes gIII (light green), which enables SP propagation.

DOI