Behavioral responses (pupil dilation, E; movement, F; and vocalizations, G) to high-force colon balloon distension in CGRP-ablated animals, compared with controls. Each point represents the average of data from an individual animal. N = 15 for controls for pupil dilation and accelerometer and 10 for vocalization; N = 10 for CGRP-ablated animals (6 of which areAdvillinCre;CalcaLSL-hDTR, DTX treated [black circles] and 4 of which areAdvillinFlp;CalcaCreER;R26FSF-LSL-Caspase3, 2 mg tamoxifen daily P21–P23 [black squares]) for pupil dilation and accelerometer; and N = 6 for CGRP-ablated animals (2 of which areAdvillinCre;CalcaLSL-hDTR, DTX treated [black circles] and 4 of which areAdvillinFlp;CalcaCreER;R26FSF-LSL-Caspase3, 2 mg tamoxifen daily P21–P23 [black squares]) for vocalization (note there are fewer animals for the vocalization data because a subset of the animals were run in a previous version of the setup with a different microphone). Comparisons for the pupil dilation and accelerometer data are paired t tests for the control, Wilcoxon matched-pairs signed rank tests for the CGRP-ablated animals, and Mann-Whitney tests for the unpaired comparisons. Comparisons for the vocalization data are Wilcoxon matched-pairs signed rank tests for the paired comparisons and a Mann-Whitney test for the unpaired comparison).
