Gel from Scientific Research

35S-methionine-labeled CCT4-3xFLAG was translated in the PURE system and the soluble and monomeric population was isolated (see Figure S5A). This was incubated with E1 and E2 enzymes, His-Ub, ATP, the indicated recombinant HERC2 variant, and ZNRD2, as indicated. The samples were analyzed directly (input) or after denaturing His-Ub pull-down (His-Ub PD).

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